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Neuraminidase Sp
规格或纯度:
Specific Activity ≥ 250 U/mg;Activity ≥ 10 U/ml
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基本描述
| 产品名称 | Neuraminidase Sp |
|---|---|
| 规格或纯度 | Specific Activity ≥ 250 U/mg;Activity ≥ 10 U/ml |
| 产品介绍 |
Product Description α(2-3) Neuraminidase Sp cleaves exclusively the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins. There is no detectable activity on α(2-6) or α(2-8) linkages or on branched α(2-3) linkages . To cleave all non-reducing terminal sialic acid residues including branched sialic acids (linked to an internal residue) from complex carbohydrates and glycoproteins, use α(2-3,6,8,9) Neuraminidase Au. Contents Included with 20 μL and 60 μL pack sizes: Specificity All non-reducing terminal branched and unbranched a-(2- 3) sialic acid. Specific Activity Assay One unit of Neuraminidase is defined as the amount of enzyme required to produce 1 μmole of methylumbelliferone in 1 minute at 37°C, pH 5.0 from MU-NANA (2’.-(4-methyl-umbelliferyl)-alpha-D-N acetylneuraminic acid]. Molecular Weight ~75,000 daltons pH optimum 6.0, active over the range 4.5-7. 50 mM sodium phosphate (pH 6.0) provides the optimal buffer for enzyme activity with sialyllactose, a standard substrate. If glycosidase treatment is performed at suboptimal pH because of glycoprotein solubility or activity requirements, expect some diminution in enzyme activity. Formulation The enzyme is provided as a sterile-filtered solution in in 50 mM Sodium phosphate pH 7.5. Stability Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity. Purity Neuraminidase Sp is tested for contaminating protease as follows: 10 μg of denatured BSA is incubated at 37°C for 24 hours with 2 μl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production host strain has been extensively tested and does not produce any detectable glycosidases. Directions for use 1. Add up to 100 μg of glycoprotein or 1 nmol of oligosaccharide to tube. 2. Add de-ionized water to a total of 14 μl. 3. Add 4 μl 5x Reaction Buffer 6.0. 4. Add 2 μl Neuraminidase Sp. 5. Incubate at 37°C for 1 hour. NOTE: longer incubation times are necessary if branched sialic acids are present. Desialylation may be monitored by SDS-PAGE if the size differential between native and de-sialylated protein is sufficient for detection.? Applications ?Structural analysis of oligosaccharides ?Determining sialic acid linkage ?Glycoprotein deglycosylation ?Removing heterogeneity from glycoproteins |
| 来源 | recombinant from Streptococcus pneumoniae in E. Coli |
产品规格参数
| 储存温度 | 2-8°C储存 |
|---|---|
| 运输条件 | 冰袋运输 |
| 酶学委员会编号 | 3.5.1.18 |
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产品问答
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