| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| L598378-30T | 30T |
期货  |
| |
| L598378-150T | 150T |
期货 |
| |
| L598378-300T | 300T |
期货 |
|
| 英文名称 | Live & deadtm animal cell viability / toxicity detection kit (calcein am, ethd-1) |
|---|---|
| 储存温度 | 避光,-20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
产品介绍： Live & DeadTM动物细胞活力/毒性检测试剂盒（Calcein AM, EthD-I）是一种为动物细胞死活检测提供双荧光染色的试剂盒。试剂盒内的两种探针可分别测定细胞内酯酶活性和质膜完整性从而反映细胞活力。本试剂盒可用于荧光显微镜、流式细胞仪、酶标仪以及其他荧光检测系统。 本试剂盒可以应用于大多数的真核哺乳动物细胞，包括贴壁细胞核的某些组织，但不适用于真菌和酵母。该试剂盒与相同用处的台盼蓝相比，更快捷安全且灵敏度更高。 产品参数： Calcein AM: Ex/Em = 494/517 nm；EthD-I: Ex/Em = 528/617 nm（结合DNA） 注意事项： 1. 使用前请将产品瞬时离心至管底，再进行后续实验。 2. 酚红或血清对于本试剂盒的检测有一定的干扰。 3. 荧光染料均存在淬灭问题，实验操作时请尽量注意避光，以减缓荧光淬灭。 4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。 应用范围： 死、活细胞染色（动物） Product introduction： Live & deadtm animal cell viability / toxicity detection kit (calcein am, ethd-i) is a kit that provides double fluorescent staining for the detection of animal cell death and survival. The two probes in the kit can respectively measure the activity of cellular lactonase and the integrity of plasma membrane to reflect cell viability. The kit can be used for fluorescence microscopy, flow cytometry, microplate reader and other fluorescence detection systems. This kit can be applied to most eukarYOtic mammalian cells, including some tissues with adherent nuclei, but it is not applicable to fungi and yeast. Compared with trypan blue, the kit is faster, safer and more sensitive. Product parameters： Calcein am: ex/em = 494 / 517 nm; Ethd-i: ex/em = 528 / 617 nm (bound DNA) Matters needing attention： 1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. phenol red or serum may interfere with the detection of this kit. 3. fluorescent dyes have quenching problems. Please try to avoid light during experimental operation to slow down fluorescence quenching. 4. for your safety and health, please wear experimental clothes and disposable gloves. Scope of application： Dead and live cell staining (animal) |
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