乳过氧化物酶 来源于牛奶
- CAS编号: 9003-99-0(milk)
- MDL号: MFCD00131484
- 酶学委员会编号: I.U.B.: 1.11.1.7
规格或纯度 | ≥35 units/mg dry weight |
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英文名称 | Lactoperoxidase from Bovine Milk |
别名 | 过氧化物酶 |
单位定义 | One Unit reduces one micromole of hydrogen peroxide per minute at 25°C, pH 6.0. Note: This unit definition is based upon a new assay method. The previous method yielded approximately 2.8 times higher results. |
生化机理 | 乳过氧化物酶催化过氧化氢与碘化物的氧化反应生成碘。该活性提供了一种温和的特异性氯胺 T 替代物,用于蛋白质和 DNA 的放射性碘化反应。 |
储存温度 | -20°C储存 |
运输条件 | 超低温冰袋运输 |
产品介绍 |
Lactoperoxidase (LPO) is a glycoprotein with a hemin prosthetic group which may occur as a mixture of two isozymes. It has a molecular weight of 77,500 daltons. LPO catalyzes the hydrogen peroxide oxidation of iodide according to the following reaction: 2I- + H2O2 + 2H+ → I2 + 2H2O. Iodide reacts directly with the heme group; upon addition of H2O2 the complex iodinates the substrate. LPO is inhibited by hydrazines. The assay procedure has been updated from that of Morrison to an ABTS?/H2O2 based method with increased sensitivity and reproducibility. Chromatographically purified. A lyophilized powder. |
酶学委员会编号 | I.U.B.: 1.11.1.7 |
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密度 | 1.047 |
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