首页Ethidium Homodimer-I （溴乙啡锭二聚体I，EthD-I）
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| 产品名称 | Ethidium Homodimer-I （溴乙啡锭二聚体I，EthD-I） |
|---|---|
| 产品介绍 |
产品介绍： Ethidium Homodimer-I (EthD-I)是一种高亲和性的荧光核酸染料，与DNA或RNA结合后，可以使荧光增强30多倍。用于哺乳动物、细菌、酵母和真菌的染色。EthD-I带有较强正电荷，所以该染料不能穿过细胞膜进行活细胞染色，但是能穿过死细胞膜的无序区域而到达细胞核并且嵌入DNA双链从而产生红色荧光。因此EthD-I可以准确的检测溶液中的核酸或者解体细胞中的核酸，是一种较灵敏的核酸染色剂。 以贴壁细胞（盖玻片）举例，每个样本100 μL染色工作液，染色工作液浓度4 μM计算，1 mg配置为工作液大概可以用于2917个样本。 产品参数： 外观：可溶于DMSO和MeOH的红色固体 Ex/Em (结合DNA）= 528/617 nm CAS号：61926-22-5 分子式：C46H50Cl4N8 分子量：856.8 注意事项： 1. 使用前请将产品瞬时离心至管底，再进行后续实验。 2. 荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。 3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。 应用范围： 细胞核染色 Product introduction： Ethidium homodimer-i (ethd-i) is a fluorescent nucleic acid dye with high affinity. After binding with DNA or RNA, it can enhance the fluorescence by more than 30 times. Used for staining of mammals, bacteria, yeast and fungi. Ethd-i has a strong positive charge, so the dye cannot cross the cell membrane for live cell staining, but it can cross the disordered region of the dead cell membrane to reach the nucleus and embed the DNA double strand to produce red fluorescence. Therefore, ethd-i can accurately detect nucleic acids in solution or in disintegrated cells, and is a more sensitive nucleic acid stain. Take adherent cells (coverslips) as an example, 100 for each sample μ L of dyeing working solution with a concentration of 4 μ M calculation, 1 mg of working fluid can be used for 2917 samples. Product parameters： Appearance: red solid soluble in DMSO and MeOH ex/em (bound DNA) = 528/617 nmcas No.: 61926-22-5 molecular formula: c46h50cl4n8 molecular weight: 856.8 Matters needing attention： 1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching. 3. for your safety and health, please wear experimental clothes and disposable gloves. Scope of application： Nuclear staining |
产品规格参数
| 储存温度 | 2-8°C储存,避光 |
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| 运输条件 | 冰袋运输 |
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产品问答
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