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Endo F2
规格或纯度:
Specific Activity >20 U/mg ;Activity >5 U/ml
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基本描述
| 规格或纯度 | Specific Activity >20 U/mg ;Activity >5 U/ml |
|---|---|
| 英文名称 | Endo F2 |
| 英文别名 | Endo-beta-N-acetylglucosaminidase F2 |
| 来源 | recombinant gene from Elizabethkingia miricola in E. Coli |
| 储存温度 | 2-8°C储存 |
| 运输条件 | 冰袋运输 |
| 产品介绍 |
Product Description Endo F2 cleaves N-linked (asparagine-linked) biantennary oligosaccharides from glycoproteins. It also will cleave high mannose glycans but at a 40x reduced rate. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. Endoglycosidase F2 is less sensitive to protein conformation than PNGase F and is therefore more suitable for deglycosylation of native proteins. However, for optimal results, denaturation of the glycoprotein is recommended. Contents Included with 20 μL and 60 μL pack sizes: Molecular weight 32,000 daltons Specific Activity Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured porcine fibrinogen in 1 minute at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved fibrinogen migrates faster). Formulation The enzyme is provided as a sterile-filtered solution in 10
mM sodium acetate, 25mM NaCl, pH 4.5 Specificity Endo F2 cleaves Asparagine-linked biantennary and high mannose glycans (at a 40X reduced rate). It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. Endoglycosidase F2 is less sensitive to protein conformation than PNGase F and is therefore more suitable for deglycosylation of native proteins. However for optimal results, denaturation of the glycoprotein is recommended. Quality & Purity Endo F2 is tested for contaminating protease as follows: 10 μg of denatured BSA is incubated at 37°C for 24 hours with 2 μl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production host strain has been extensively tested and does not produce any detectable glycosidases. Stability Several days exposure to ambient temperatures will not
reduce activity. Stable at least 12 months when stored
properly. Directions for use 1. Add up to 200 μg of glycoprotein to an Eppendorf tube. Adjust to 38 μl final volume with de-ionized water. 2. Add 10 μl 5x Reaction Buffer 4.5 3. Add 2.0 μl of Endo F2 to the reaction. Incubate 1 hour at 37°C. Monitor cleavage by SDS-PAGE The production host strain has been extensively tested and does not produce any detectable glycosidases. |
名称和标识符
| 酶学委员会编号 | 3.2.1.96 |
|---|
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