Dilinoleyl DiI(细胞膜橙红色荧光探针)
- 分子式: C59H89ClN2O4
- 分子量: 925.80
英文名称 | Dilinoleyl DiI (cell membrane orange red fluorescent probe) |
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储存温度 | 2-8°C储存,避光 |
运输条件 | 冰袋运输 |
产品介绍 |
Dilinoleyl DiI也被称为FAST DiI,与DiI有相同的吸收和发射波长。由于Dlinoleyl DiI中含有不饱和烃链,使Dilinoleyl DiI比DiI在细胞膜上的横向扩散速度更快。正是由于其这个特性,使其广泛应用于神经元组织的追踪。 Dilinoleyl DiI染色后可进行多聚甲醛(不可使用甲醇等其他试剂)的固定,但不建议在染色后进行透化的过程。此外,在固定透化(室温下用0.1% TritonX-100透化)后,也可以很好地进行质膜染色。 以每次使用100 μL染色工作液,染色工作液浓度10 μM计算,5 mg配置为工作液大概可以用5400次。 产品参数: Ex/Em (MeOH) = 549/565 nm 注意事项: 1. 使用前请将产品瞬时离心至管底,再进行后续实验。 2. Dilinoleyl DiI染色固定的细胞或组织样品时,通常使用配制在PBS中的4%多聚甲醛进行固定,使用其它不适当的固定液会导致荧光背景较高。 3. 荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。 4. 为了您的安全和健康,请穿实验服并戴一次性手套操作。 应用范围: 细胞膜荧光染料;神经元顺行和逆行示踪;细胞长期示踪 使用方法: ?Dilinoleyl DiI, also known as fast DiI, has the same absorption and emission wavelength as DiI. Because dlinoleyl DiI contains unsaturated hydrocarbon chains, the lateral diffusion speed of dilinoleyl DiI on the cell membrane is faster than that of DiI. Because of this property, it is widely used in tracking neuronal tissues. After dilinoleyl DiI staining, paraformaldehyde (methanol and other reagents cannot be used) can be fixed, but permeabilization after staining is not recommended. In addition, after fixed permeabilization (permeabilization with 0.1% TritonX-100 at room temperature), the plasma membrane staining can also be well performed. At 100 per use μ L of dyeing working solution with a concentration of 10 μ According to m calculation, 5 mg of working solution can be used for 5400 times. Product parameters: Ex/Em (MeOH) = 549/565 nm Matters needing attention: 1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. when dilinoleyl DiI is used to stain fixed cells or tissue samples, 4% paraformaldehyde prepared in PBS is usually used for fixation. The use of other inappropriate fixatives will lead to high fluorescence background. 3. fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching. 4. for your safety and health, please wear experimental clothes and disposable gloves. Scope of application: Cell membrane fluorescent dye ; anterograde and retrograde tracing of neurons ; long-term cell tracing Usage: |
分子量 | 925.80 |
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溶解性 | 可溶于乙醇、DMSO和DMF |
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