首页
400-620-6333
切换导航
搜索
基本描述
| 规格或纯度 | 10000× in DMSO |
|---|---|
| 英文名称 | DuGreen nucleic acid dye |
| 储存温度 | 2-8°C储存,避光,干燥 |
| 运输条件 | 冰袋运输 |
| 产品介绍 |
产品描述 DuGreen与Super GelGreen一样也是一种油性大分子电(分子量>1000)，是新型无毒核酸染料。这种独特的油性大分子，分子量>1000,?不易挥发升华、不易吸入人体，不能穿透细胞膜进入活体细胞内，且在凝胶染色浓度下没有诱变性，具有使用安全、检测灵敏等特点，可以作为各种核酸电泳的染色剂，适用于各种片段大小染色。与标准凝胶成像系统和可见光激发的凝胶观察装置完美兼容，适用于紫外凝胶成像系统或蓝色可见光激发的凝胶观察装置。本公司提供的DuGreen?荧光染料为浓缩的10,000.染料。 产品特点 1.安全无毒：独特的油性大分子特点使其不能穿透细胞膜进入细胞内，该染料的诱变性远小于EB。 2.灵敏度高：适用于各种大小片段的电泳染色，对核酸迁移的影响较小。 3.稳定性高：适用于使用微波或其它加热方法制备琼脂糖凝胶；室温下在酸或碱缓冲液中极其稳定，耐光性强。 4.信噪比高：样品荧光信号强，背景信号低。 5.操作简单：在预制胶和电泳过程中不降解,可直接用可见光凝胶透射仪观察。 6.适用范围广：可选择电泳前染色（胶染法）或电泳后染色（泡染法）；适用琼脂糖凝胶或聚丙烯酰胺凝胶电泳；可用于dsDNA、ssDNA 或RNA 染色。 7.完美兼容：适用于使用254nm 激发的紫外凝胶成像系统或蓝色可见光激发的凝胶观察装置。它和SYBR Green I的光谱相似，灵敏度相当，但更加稳定。 储存条件：?2-8℃避光干燥可保存12个月。
一、琼脂糖凝胶电泳染色 将DuGreen Nucleic Acid Dye加入凝胶中 1．制胶：按常规操作，制备琼脂糖凝胶，加入浓缩的10,000xDuGreen，使其在凝胶中的终浓度为1x（例如：制备50ml的凝胶，加入染料5μl），轻轻摇匀，倒胶。 2．按常规方法电泳，观测结果。 二、泡染法?? （1）按照以上常规方法进行电泳。?用于胶回收等高浓度DNA样品强烈推荐泡染法！ （2）将DuGreen10,000×储液稀释约3,300倍到0.1M NaCl溶液中制成3×染色液。例如将15μL DuGreen10,000×原装液加入到50mL 0.1M NaCl溶液中。 （3）将凝胶小心地放入合适的容器中(如聚丙烯容器中)缓慢加入足量的3×?染色液浸没凝胶。室温振荡染色30min左右，最佳染色时间根据凝胶厚度以及琼脂糖浓度不同而略有不同。对于含1％的凝胶，染色时间约30min。? （4）用302nm激发的紫外凝胶成像系统观察结果。 ? 注意事项：请务必在使用本试剂盒之前阅读此注意事项。 1．由于DuGreen??具有良好的热稳定性，可以在热的琼脂糖溶液中直接添加，而不需要等待溶液冷却。摇晃，振荡或者翻转以保证染料充分混匀。也可以选择将DuGreen??储液加到琼脂糖粉末和电泳缓冲液中，然后用微波炉或其他常用方式加热以制备琼脂糖凝胶。DuGreen??兼容所有常用的电泳缓冲溶液。 2．如果条带总是弥散或分离不理想，请使用泡染法染色以确认问题是否与染料有关。如果染色后问题依旧存在，则说明问题与染料无关，请尝试：降低琼脂糖浓度；选用更长的凝胶；延长凝胶时间以保证边缘清晰；改进上样技巧或选择泡染法染色。 3．DuGreen?对玻璃器皿和非聚丙烯材料具有一定的亲合力。建议在稀释、贮存、染色等使用过程中用聚丙烯类容器。 4．对于聚丙烯酰胺凝胶请使用泡染法。 Product description DuGreen is a kind of oily macromolecule like Super GelGreen (molecular weight>1000), and it is a new type of non-toxic nucleic acid dye. This unique oily macromolecule has a molecular weight of >1000, is not easy to volatilize and sublimate, is difficult to inhale, cannot penetrate cell membranes into living cells, and has no mutagenicity at the gel staining concentration. It has the characteristics of safety in use and sensitive detection. It can be used as a staining agent for various nucleic acid electrophoresis, suitable for staining of various fragment sizes. It is perfectly compatible with standard gel imaging systems and gel observation devices excited by visible light, and is suitable for ultraviolet gel imaging systems or gel observation devices excited by blue visible light. The DuGreen? fluorescent dyes provided by our company are concentrated 10,000. dyes. ? Features 1. Safe and non-toxic: The unique oily macromolecule characteristics make it unable to penetrate the cell membrane and enter the cell. The mutagenicity of this dye is far less than EB. 2. High sensitivity: It is suitable for electrophoretic staining of fragments of various sizes, with less impact on nucleic acid migration. 3. High stability: suitable for preparing agarose gel using microwave or other heating methods; it is extremely stable in acid or alkali buffer at room temperature, and has strong light resistance. 4. High signal-to-noise ratio: the sample has strong fluorescence signal and low background signal. 5. Simple operation: it does not degrade during the precast gel and electrophoresis process, and can be directly observed with a visible light gel transmission instrument. 6. Wide range of application: you can choose staining before electrophoresis (gel staining method) or staining after electrophoresis (bubble staining method); suitable for agarose gel or polyacrylamide gel electrophoresis; can be used for dsDNA, ssDNA or RNA staining. 7. Perfect compatibility: Suitable for use with 254nm excitation ultraviolet gel imaging system or blue visible light excitation gel observation device. It has a spectrum similar to SYBR Green I, with similar sensitivity, but more stable. Storage conditions: 2-8℃, dark and dry, can be stored for 12 months. ? Steps 1. Agarose gel electrophoresis staining Add DuGreen Nucleic Acid Dye to the gel ①. Gel preparation: According to the usual operation, prepare agarose gel, add concentrated 10,000xDuGreen to make the final concentration in the gel 1x (for example: prepare 50ml gel, add 5μl dye), shake gently, pour glue. ②. Perform electrophoresis according to conventional methods and observe the results. 2. Bubble dyeing method (1) Perform electrophoresis according to the above conventional methods. The bubble staining method is strongly recommended for high-concentration DNA samples such as gel recovery! (2) Dilute DuGreen10,000× stock solution about 3,300 times to 0.1M NaCl solution to make 3× staining solution. For example, add 15μL DuGreen10,000× original solution to 50mL 0.1M NaCl solution. (3) Put the gel carefully into a suitable container (such as a polypropylene container) and slowly add enough 3× staining solution to submerge the gel. Shake and stain at room temperature for about 30 minutes. The optimal staining time varies slightly depending on the thickness of the gel and the concentration of agarose. For a gel containing 1%, the staining time is about 30 minutes. To (4) Observe the results with a UV gel imaging system excited at 302nm. ? Note: Please be sure to read this note before using this kit. 1. Due to its good thermal stability, DuGreen? can be added directly to the hot agarose solution without waiting for the solution to cool. Shake, shake or invert to ensure that the dye is well mixed. You can also choose to add DuGreen? stock solution to agarose powder and running buffer, and then heat it in a microwave oven or other common methods to prepare agarose gel. DuGreen? is compatible with all commonly used running buffer solutions. 2. If the bands are always dispersed or unsatisfactory, please use the foam dyeing method to confirm whether the problem is related to the dye. If the problem persists after staining, it means that the problem has nothing to do with the dye. Please try: reduce the agarose concentration; use a longer gel; extend the gel time to ensure a clear edge; improve the sample loading technique or choose the dyeing method. 3. DuGreen? has a certain affinity for glassware and non-polypropylene materials. It is recommended to use polypropylene containers during dilution, storage, and dyeing. 4. For polyacrylamide gel, please use the foam dyeing method. |
化学和物理性质
| 敏感性 | 对光和湿度敏感 |
|---|
产品问答
产品问答
上海阿拉丁生化科技股份有限公司
? 2016 ALADDIN-E.COM . 版权所有,未经授权禁止拷贝本站资料,如有违反,将追究法律责任
危险品化学品经营许可证（带存储）