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基本描述
| 规格或纯度 | 10000× in DMSO |
|---|---|
| 英文名称 | DuFinder nucleic acid dye |
| 储存温度 | 2-8°C储存,避光,干燥 |
| 运输条件 | 冰袋运输 |
| 产品介绍 |
本品用DMSO溶解，因DMSO的熔点是18.5℃，使用前请放置到室温充分溶解。 DuFinder产品简介 Dufinder?是一种新型核酸染料，具有安全、灵敏等突出优点，可以代替溴化乙锭（EB）作为各种核酸电泳的染色剂。与强致癌性的EB不同，Dufinder?属于花箐类染料，毒性很低，使用安全,可有效保护实验操作者和环境。 DuFinder?产品参数 Ex(nm) 508????Em(nm) 533? ? Solvent: DMSO DuFinder产品特点 1.?安全：Dufinder??染料为花箐类染料，其致突变性远低于EB数倍甚至数十倍。 2.?灵敏：?检测核酸的灵敏度平均高于EB染色法10倍左右。 3.?经济：?价格低于进口同类产品5～10倍。 4.?操作简单：?本染料有多种使用方法，操作简单?。 5.?通用性：?适用于多种核酸电泳分析。可以对DNA、RNA、及寡核苷酸等进行染色。 6.?兼容性：?对常用酶（如：Taq?酶、逆转录酶、内切酶、连接酶等）没有抑制作用。 DuFinder使用方法 方法一：胶染法（用法同EB）（推荐方法） （1）?制胶时加入Dufinder?核酸染料。（例如：每50mL?琼脂糖溶液中加入5μL Dufinder? 10,000×?储液，以此比例类推）。 （2）?按照常规方法进行电泳。 此方法染色染料用量相对较少。500 μL染料大约可以做100块?50mL的胶。 方法二：泡染法 （1）?按照常规方法进行电泳。 （2）?用PH 7.0～8.5?的缓冲液（如：TAE，TBE?或?TE），按照10000：1（或10000：2）的比例稀释Dufinder染料浓缩液，混匀，制成染色溶液，。 （3）?将染色溶液倒入合适的聚丙烯容器中，放入凝胶，用铝箔等盖住容器使染料避光。室温振荡染色10～45分钟，染色时间因凝胶浓度和厚度而定。凝胶越厚、浓度越高，所需染色时间越长。也可以在聚丙烯酰胺凝胶上直接染色（玻璃平皿必须预先经过硅烷化溶液处理，避免染料吸附在玻璃表面上）。将配好的工作溶液轻轻地倒在胶板上，让工作液均匀地覆盖整个胶板，并染色10～30分钟。 （4）?观测。 DuFinder注意事项及其他说明 1.?该染料为花箐类染料，没有致癌性，但有可能会刺激皮肤和眼睛，操作时应戴手套。 2.?染料从冰箱中取出后应恢复至室温，使溶液充分融化，并混匀。 3.?在常规用酒精沉淀核酸的过程中，Dufinder可以全部从双链核酸上去掉。 4.?Dufinder对玻璃和非聚丙烯材料具有一定亲合力。建议在稀释、贮存、染色等使用过程中用聚丙烯类容器。 5.?储存：储存于4℃，有效期至少一年。 6.?本品仅供科研使用。 This product is dissolved in DMSO. Because the melting point of DMSO is 18.5℃, please put it to room temperature to dissolve fully before use. DuFinder product introduction Dufinder is a new type of nucleic acid dye with outstanding advantages such as safety and sensitivity. It can replace ethidium bromide (EB) as a dye for various nucleic acid electrophoresis. Different from EB, which is highly carcinogenic, Dufinder is a kind of dye that has very low toxicity and is safe to use. It can effectively protect experiment operators and the environment. The fluorescence signal of Dufinder combined with dsDNA can be enhanced by 800-1000 times, and the sensitivity of detecting nucleic acid is about 10 times higher than that of EB staining method on average. The Dufinder dye has a maximum absorption peak of 470nm, and the nucleic acid bound to the dye exhibits green fluorescence. Observe with a visible light gel transmissometer. UV gel transmissometer can also be used for observation, but the effect is slightly worse. To DuFinder product parameters Ex(nm) 508? ? ? Em(nm) 533? ? ? Solvent: DMSO DuFinder product features 1. Safety: Dufinder dyes are Huaqing dyes, and their mutagenicity is much lower than EB several times or even dozens of times. 2. Sensitivity: The sensitivity of nucleic acid detection is about 10 times higher than EB staining method on average. 3. Economy: The price is 5-10 times lower than that of imported similar products. 4. Simple operation: There are many ways to use this dye, and the operation is simple. 5. Versatility: Suitable for a variety of nucleic acid electrophoresis analysis. It can stain DNA, RNA, and oligonucleotides. 6. Compatibility: It has no inhibitory effect on commonly used enzymes (such as Taq enzyme, reverse transcriptase, endonuclease, ligase, etc.). How to use DuFinder Method 1: Paste dyeing method (the usage is the same as EB) (recommended method) (1) Add Dufinder nucleic acid dye when making gel. (For example: add 5μL Dufinder 10,000× stock solution to every 50mL agarose solution, and analogy with this ratio). (2) Perform electrophoresis in accordance with conventional methods. This method uses relatively less dyes. 500 μL of dye can make about 100 pieces of 50mL glue. Method 2: Bubble dyeing method (1) Perform electrophoresis in accordance with conventional methods. (2) Dilute the Dufinder dye concentrate at a ratio of 10000:1 (or 10000:2) with a buffer solution of pH 7.0～8.5 (such as TAE, TBE or TE) and mix it to make a dyeing solution. (3) Pour the dye solution into a suitable polypropylene container, put the gel, and cover the container with aluminum foil to protect the dye from light. Shake and stain at room temperature for 10 to 45 minutes. The staining time depends on the gel concentration and thickness. The thicker the gel and the higher the concentration, the longer the dyeing time required. It can also be dyed directly on the polyacrylamide gel (the glass plate must be treated with a silanization solution in advance to prevent the dye from being adsorbed on the glass surface). Pour the prepared working solution gently on the rubber plate, let the working solution evenly cover the entire rubber plate, and dye for 10-30 minutes. (4) Observation. DuFinder notes and other instructions 1. The dye is a flower qing dye, which is not carcinogenic, but it may irritate the skin and eyes. Wear gloves when handling it. 2. After the dye is taken out of the refrigerator, return to room temperature, so that the solution is fully melted, and mixed. 3. In the process of conventional alcohol precipitation of nucleic acids, Dufinder can remove all double-stranded nucleic acids. 4. Dufinder has a certain affinity for glass and non-polypropylene materials. It is recommended to use polypropylene containers during dilution, storage, and dyeing. 5. Storage: Store at 4°C, valid for at least one year. 6. This product is for scientific research use only. |
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