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基本描述
| 英文名称 | DRAQ7 Fluorescent Probe |
|---|---|
| 储存温度 | 2-8°C储存,避光,干燥 |
| 运输条件 | 冰袋运输 |
| 产品介绍 |
产品介绍 1.?规格：0.3mM?0.5mL,1mL 2.?参数：MW=412.49；Wavelength:Ex/Em=633/695nm；Solvent: Water 3.?储存：4?℃避光保存2年 产品描述 DRAQ7是一种远红光的DNA染料，能够染色死亡和透化细胞中的细胞核。由于它对活细胞是非渗透性的， 所以可用于区分活细胞和死细胞，是研究死亡或膜受损细胞 的理想工具，并且可以快速染色死亡或透化细胞的dsDNA /细胞核。它可用于大多数细胞类型，真核和原核生物：哺乳 动物，细菌，寄生虫，植物等，可与活细胞染料共同使用， 并用于siRNA?研究和其他动态活性检测。 DRAQ7是PI?和7-AAD?的理想替代品，因为它不受紫外线的激发，并且与PE以及PE同系物没有发射重叠，可与FITC、PE?和其他紫色染料结合用于多色分析，无需洗涤或RNase?处理。DRAQ7可用流式细胞仪，激光扫描细胞仪和 共聚焦显微镜进行检测DRAQ7在647nm?处被最佳激发，使用流式细胞仪的 时候可以使用488nm，514nm?和568 nm?波长激发。对于成像显微术，建议使用633?或647 nm?的光源进行激发。由于它的发射和激发波长范围很宽，不建议将DRAQ7与其他可被488?或633 nm?激发的远红光荧光染料联用。 操作说明 ①. 准备不含有叠氮化钠的PBS?缓冲液。 ②. 固定细胞：4%?多聚甲醛的PBS?在室温下固定15 min。 ③. 用PBS?冲洗细胞两次。 ④. 将细胞在0.5% Triton X-100?的PBS?中室温透化10 min。 ⑤. 用PBS?冲洗细胞两次。 ⑥. 可选：根据您的标准进行免疫荧光染色操作。 ⑦. 根据不同细胞将DRAQ7稀释至最佳浓度，室温染色5-30 min（37℃?染色更快，可能需要更短的染色时间）。 建议稀释倍数在1：15?至1：200?之间。 ⑧.?用荧光显微镜，流式细胞仪等检测远红外细胞核染色。 注意事项 1. 荧光染料存在淬灭问题，请尽量注意避光，以减缓淬灭。 2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。 Product introduction 1. Specifications: 0.3mM 0.5mL, 1mL 2. Parameters: MW=412.49; Wavelength: Ex/Em=633/695nm; Solvent: Water 3. Storage: 4 ℃ protected from light for 2 years Product description DRAQ7 is a far-red DNA dye that can stain nuclei in dead and permeabilized cells. Because it is impermeable to living cells, it can be used to distinguish between living cells and dead cells. It is an ideal tool for studying dead or membrane-damaged cells, and it can quickly stain the dsDNA/nucleus of dead or permeabilized cells. It can be used in most cell types, eukaryotic and prokaryotic organisms: mammals, bacteria, parasites, plants, etc. It can be used with live cell dyes and used for siRNA research and other dynamic activity detection. DRAQ7 is an ideal substitute for PI and 7-AAD because it is not excited by ultraviolet rays and has no emission overlap with PE and PE homologues. It can be combined with FITC, PE and other purple dyes for multicolor analysis without washing or RNase treatment. DRAQ7 can be detected by flow cytometry, laser scanning cytometer and confocal microscope. DRAQ7 is optimally excited at 647nm. When using flow cytometry, excitation at 488nm, 514nm and 568nm can be used. For imaging microscopy, it is recommended to use a light source of 633 or 647 nm for excitation. Due to its wide emission and excitation wavelength range, it is not recommended to combine DRAQ7 with other far-red fluorescent dyes that can be excited at 488 or 633 nm. Instructions ①. Prepare PBS buffer without sodium azide. ②. Fix the cells: fix in 4% paraformaldehyde in PBS for 15 min at room temperature. ③. Wash the cells twice with PBS. ④. Permeabilize the cells in 0.5% Triton X-100 in PBS for 10 min at room temperature. ⑤. Wash the cells twice with PBS. ⑥. Optional: Perform immunofluorescence staining operations according to your standards. ⑦. Dilute DRAQ7 to the best concentration according to different cells, and stain at room temperature for 5-30 min (37℃ staining is faster, and shorter staining time may be required). The recommended dilution factor is between 1:15 and 1:200. ⑧. Detect far-infrared cell nuclear staining with fluorescence microscope, flow cytometer, etc. Precautions 1. Fluorescent dyes have quenching problems, please try to avoid light to slow down quenching. 2. For your safety and health, please wear lab coats and disposable gloves. |
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