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DNA酶I 来源于牛胰腺(重组)

规格或纯度: ≥5000 units/mg protein
有货

库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
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基本描述

规格或纯度 ≥5000 units/mg protein
英文名称 Deoxyribonuclease I from bovine pancreas(Recombinant)
别名 脱氧核糖核酸酶 I
英文别名 Deoxyribonucleate 5′-oligonucleotido-hydrolase;DNase I
生化机理 DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)6 and actin7 are known to inhibit the enzyme activity.
储存温度 2-8°C储存
运输条件 冰袋运输
备注 卖完停产，不再备货
产品介绍

用于从蛋白质样品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drμgs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

名称和标识符

EC号 232-667-0
分子式


PubChem CID 597667

化学和物理性质

敏感性 易潮湿

安全信息

WGK Germany 3
RTECS RF0750000
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

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