| 产品名称 | HT-29 Whole Cell Lysate |
|---|---|
| 规格或纯度 | Mycoplasma free |
| 产品介绍 |
The HT-29 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process HT-29 cells are human intestinal epithelial cells which produce the secretory component of Immunoglobulin A (IgA), and carcinoembryonic antigen (CEA). Cells are used for tumourigenicity studies. |
| 应用 | WB |
|---|---|
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4). |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 μg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white po |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
HT-29 Whole Cell Lysate (cl155904) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)
?
Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
HT-29 Whole Cell Lysate (cl155904) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)
?
Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
HT-29 Whole Cell Lysate (cl155904) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)
?
Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
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