| 产品名称 | LNCaP Whole Cell Lysate |
|---|---|
| 规格或纯度 | Mycoplasma free |
| 产品介绍 |
Product Description： The LNCaP whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. The LNCaP-FGC (fast growing colony) cell line is a subline derived from the LNCaP cell line, sharing all the main characteristics, including its androgen sensitivity, described for the parental line. These fibroblast-like cells express high levels of human prostatic acid phosphatase, androgen receptors and prostate antigen. They are used as a model of prostate cancer especially in studies of the development of androgen resistance in human prostate cancer. |
| 应用 | WB |
|---|---|
| 物理外观 | Lyophilized |
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail(EDTA-Free Protease Inhibitor Cocktail ) and phosphatase inhibitor(Na3VO4). |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 μg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
LNCaP Whole Cell Lysate (cl155902) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897)
Lane 2: MOLT-4 Whole Cell Lysate (cl155905)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
LNCaP Whole Cell Lysate (cl155902) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897)
Lane 2: MOLT-4 Whole Cell Lysate (cl155905)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
LNCaP Whole Cell Lysate (cl155902) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897)
Lane 2: MOLT-4 Whole Cell Lysate (cl155905)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
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