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A-431 Whole Cell Lysate
规格或纯度:
Mycoplasma free
- 应用: WB
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基本描述
| 产品名称 | A-431 Whole Cell Lysate |
|---|---|
| 规格或纯度 | Mycoplasma free |
| 产品介绍 |
Product Description： The A-431 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. A-431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK. |
产品规格参数
| 应用 | WB |
|---|---|
| 物理外观 | Lyophilized |
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4). |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 μg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
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