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NIH/3T3 Whole Cell Lysate
规格或纯度:
200μg, Mycoplasma free
- 应用: WB
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基本描述
| 产品名称 | NIH/3T3 Whole Cell Lysate |
|---|---|
| 规格或纯度 | 200μg, Mycoplasma free |
| 产品介绍 |
Product Description： The NIH/3T3 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. NIH/3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is therefore used for DNA transfection studies. |
产品规格参数
| 应用 | WB |
|---|---|
| 物理外观 | Lyophilized |
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4). |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 μg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
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