| 产品名称 | MCF7 Whole Cell Lysate |
|---|---|
| 规格或纯度 | 200μg, Mycoplasma free |
| 产品介绍 |
Product Description： The MCF7 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin. |
| 应用 | WB |
|---|---|
| 物理外观 | Lyophilized |
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4). |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 μg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white po |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
MCF7 Whole Cell Lysate (cl155899)- Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: MCF7 Whole Cell Lysate (cl155899)
Lane 3: HeLa Whole Cell Lysate (cl155894)
Lane 4: Hep G2 Whole Cell Lysate (cl155896)
Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
MCF7 Whole Cell Lysate (cl155899) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: MCF7 Whole Cell Lysate (cl155899)
Lane 3: HeLa Whole Cell Lysate (cl155894)
Lane 4: Hep G2 Whole Cell Lysate (cl155896)
Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
MCF7 Whole Cell Lysate (cl155899) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: MCF7 Whole Cell Lysate (cl155899)
Lane 3: HeLa Whole Cell Lysate (cl155894)
Lane 4: Hep G2 Whole Cell Lysate (cl155896)
Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
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400-620-6333
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