| 产品名称 | HEK-293 Whole Cell Lysate |
|---|---|
| 规格或纯度 | 200μg, Mycoplasma free |
| 产品介绍 |
Product Description： This HEK-293 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. Human Embryonic Kidney (HEK) 293 cells were originally derived from human embryonic kidney and subsequently demonstrated to be a useful cell type to produce adenovirus, other viral vectors, and effectively glycosylated human recombinant proteins. They are therefore used for virology studies and transfected to express recombinant proteins for studies in a number of research fields. |
| 应用 | WB |
|---|---|
| 物理外观 | Lyophilized |
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4). |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 μg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
HEK-293 Whole Cell Lysate (cl155895) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
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Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3:?A431 Whole Cell Lysate (cl155901)
Lane 4:?NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5:?HT-29 Whole Cell Lysate (cl155904)
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Lysates at 20 μg per lane
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Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
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Predicted band size: 42 kDa
Observed band size:?42 kDa
HEK-293 Whole Cell Lysate (cl155895) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate?(cl155904)
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Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
HEK-293 Whole Cell Lysate (cl155895) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
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