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10×Capping Reaction Buffer

规格或纯度: 医药级，10×

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
C489098-1ml	1ml	现货
C489098-5ml	5ml	现货
C489098-10ml	10ml	现货
C489098-50ml	50ml	期货

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基本描述

规格或纯度

医药级，10×

英文名称

10×Capping Reaction Buffer

储存温度

-20°C储存

运输条件

超低温冰袋运输

产品介绍

产品描述

10×Capping Reaction Buffer 是牛痘病毒加帽酶（GMP-M062）和 mRNA Cap 2’-O 甲基转移酶（GMP-M072）的配套反应缓冲液。1×Capping Reaction Buffer 配方为：50mM Tris-HCl（pH 8.0 at 25℃），5mM KCl，1mM DTT，1mM MgCl₂。?

本制品采用药用规格原辅料生产，并严格控制生产过程中各类污染等，符合 GMP 规范的产品生产与质量管理规程保障生产过程及所有原辅料可追溯。

质量要求

项目	标准
外观	澄明液体
可见异物	符合规定
pH值	7.5-8.5
核酸内切酶残留	底物的降解不超过10%
核酸外切酶残留	底物的降解不超过10%
RNA酶残留	底物的降解不超过10%
细菌内毒素含量	≤1EU/ml
支原体检测	阴性
重金属残留	≤10ppm

遵循以下规范生产

1. ISO 9001:2015, certified facility。

2. 《GMP 附录-细胞治疗产品》国家药品监督管理局。

3. 《人用基因治疗总论-中国药典 2020》国家药典委。

4. USP Chapter <1043>, Ancillary Materials for Cell, Gene, and Tissue-Engineered Products 用于细胞治疗，基因治疗和组织工程产品中的辅料。

5. USP Chapter <92>, Growth Factors and Cytokines Used in Cell Therapy Manufacturing 细胞治疗产品生产过程中细胞因子和生长因子。

6. Ph. Eur. General Chapter 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products 用于生产细胞或基因治疗药物的生物来源原料。

产品特点

通用性：适用于 Cap0 和 Cap1 RNA 合成的酶促加帽反应。?

高效性：本体系可用于 T7 RNA Polymerase反应产生的 RNA 的加帽反应，使加帽反应在一小时之内完成，效率接近 100%，并且保证正确的方向。

保存温度

-20±5 ℃。

注意事项??

1. 使用时请上下颠倒轻轻混合，避免起泡，并经轻微离心后使用。反应液的配制、分装请使用新的（无RNA 酶污染的）枪头、Microtube等，确保所有试剂中无 RNA 酶污染。?

2. 为保证反应有效进行，请在冰上操作。

3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

Product Description

ten × Capping reaction buffer is a matching reaction buffer for vaccinia virus capping enzyme (gmp-m062) and mRNA cap 2 '-o methyltransferase (gmp-m072). one × The formula of capping reaction buffer is: 50mm Tris HCl (pH 8.0 at 25 ℃), 5mm KCl, 1mm DTT, 1mm MgCl₂.

This product is produced with raw and auxiliary materials of medicinal specifications, and all kinds of pollution in the production process are strictly controlled. Product production and quality management procedures in line with GMP specifications ensure that the production process and all raw and auxiliary materials can be traced.

Quality requirements

project?	standard
appearance?	Clear liquid
Visible foreign matter?	Compliance with regulations
PH value?	7.5-8.5
Endonuclease residues?	Degradation of substrate shall not exceed 10%
Exonuclease residues?	Degradation of substrate shall not exceed 10%
RNase residue?	Degradation of substrate shall not exceed 10%
Bacterial endotoxin content?	≤1EU/ml
Mycoplasma detection?	negative
Heavy metal residues?	≤10ppm

Follow the following specifications

1. ISO 9001:2015, certified facility。

2. GMP appendix - cell therapy products State Drug Administration.

3. general introduction to human gene therapy - Chinese Pharmacopoeia 2020, National Pharmacopoeia Committee.

4. USP chapter <1043>, adjuvant materials for cell, gene, and tissue engineered products.

5. USP chapter <92>, growth factors and cytokines used in cell therapy manufacturing.

6. Ph. Eur.? General chapter 5.2.12, raw materials of biological origin for the production of cell-based and gene therapy medical products.

Product features

Versatility: it is applicable to the enzymatic capping reaction of cap0 and cap1 RNA synthesis.

Efficiency: this system can be used for the capping reaction of RNA produced by T7 RNA polymerase ?reaction, so that the capping reaction can be completed within one hour, the efficiency is close to 100%, and the correct direction can be ensured.

Storage temperature

-20±5 ℃。

Matters needing attention

1. when using, please mix it upside down gently to avoid foaming, and use it after slight centrifugation. For the preparation and sub packaging of reaction solution, please use new (non RNase contaminated) gun heads, microtubes, etc. to ensure that all reagents are free of RNase contamination.

2. to ensure effective reaction, please operate on ice.

3. for your safety and health, please wear experimental clothes and disposable gloves.

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10×Capping Reaction Buffer

库存信息

库存信息

库存信息

库存信息

基本描述

相关文档

质检报告COA

产品问答

产品问答

91高清自产国产拍,无码免费A级毛片大全,日本欧美国产中文字幕,99re热视频这里只有精品视频首页

91高清自产国产拍,无码免费A级毛片大全,日本 欧美 国产中文字幕,99re热视频这里只有精品视频首页

10×Capping Reaction Buffer

库存信息

库存信息

库存信息

库存信息

基本描述

相关文档

质检报告COA

产品问答

产品问答

91高清自产国产拍,无码免费A级毛片大全,日本 欧美 国产中文字幕,99re热视频这里只有精品视频首页

91高清自产国产拍,无码免费A级毛片大全,日本欧美国产中文字幕,99re热视频这里只有精品视频首页

91高清自产国产拍,无码免费A级毛片大全,日本欧美国产中文字幕,99re热视频这里只有精品视频首页