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C3b (Rat)
规格或纯度:
1.0 mg/mL, 0.22 μm filtered
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基本描述
| 产品名称 | C3b (Rat) |
|---|---|
| 规格或纯度 | 1.0 mg/mL, 0.22 μm filtered |
| 产品介绍 |
Protein Purity ≥85% by SDS PAGE Extinction Coeff A280 nm = 1.016 at 1.0 mg/ml for pure C3 Molecular Weight 175,000 Da (2 chains) General Description Rat C3b is derived from native purified rat C3 upon cleavage and?release of C3a with the alternative pathway C3 convertase. C3 is central to the activation of all?three pathways of complement activation (Law, S.K.A. and Reid, K.B.M. (1995)). Initiation of?each pathway generates proteolytic enzyme complexes (C3 convertases) which are bound to the?target surface. These enzymes cleave a peptide bond in C3 releasing the anaphylatoxin C3a and?activating C3b. For a brief time (~60 μs) this nascent C3b is capable of reacting with and?covalently coupling to hydroxyl groups on the target surface. Carbohydrates are the favored?target, but protein hydroxyls and amino groups also react. This process of tagging the target?surface with C3b is called opsonization. The reactive site in nascent C3b is a thioester (Tack B.J.,?et al. (1980); Pangburn M.K. and MüllerEberhard H.J. (1980)) and C3b is linked to the target?through a covalent ester bond (an amide bond is formed if C3b is attached to amino groups).?Most of the C3 activated during complement activation never attaches to the surface because its?thioester reacts with water forming fluid phase C3b which is rapidly inactivated by factors H and?I forming iC3b. Surface-bound C3b is necessary in all three pathways for efficient activation of?C5 and formation of C5b-9 complexes that lyse the target cell membrane. Surface-bound C3b?and its breakdown products iC3b and C3d are recognized by numerous receptors on lymphoid?and phagocytic cells which use the C3b ligand to stimulate antigen presentation to cells of the?adaptive immune system. The end result is an expansion of target-specific B-cell and T-cell?populations. Physical Characteristics & Structure Rat C3b is composed of two disulfide-linked chains. Analysis of purified rat C3b by?SDS/polyacrylamide gel electrophoresis (Invitrogen) under non-reduced conditions shows the? mobility of rat C3b to be similar to that of human C3b (176,000 daltons). Under reduced?conditions, the migration of the alpha prime chain (α’-chain) of rat C3b is similar to that of?human C3b alpha prime chain (101,000 daltons) while the beta chain migrates as a 62,000 dalton?band versus the human C3b beta chain (75,000 daltons).The concentration of purified rat C3b is determined by using the calculated extinction?coefficient of rat C3 (E1%/280nm = 10.16) based on its amino acid sequence using ProtParam and?assumes all pairs of Cys residues form cystines (i.e. a pair of cystine molecules are joined by a?disulfide bond). Function The biological functions of C3 are described above in the General Description section. Precautions/Toxicity/Hazards This protein is purified from animal plasma/serum and therefore precautions appropriate? for handling any animal blood-derived product must be used. References Law, S.K.A. and Reid, K.B.M. (1995) Complement 2nd Edition (ISBN 0199633568) Oxford?University Press, Oxford. Tack BF, Harrison RA, Janatova J, Thomas ML, Prahl JW. (1980) Evidence for presence of an?internal thiolester bond in third component of human complement. Proc Natl Acad Sci U S A.?77:5764-8. Pangburn M.K. and Müller-Eberhard H.J. (1980) Relation of putative thioester bond in C3 to?activation of the alternative pathway and the binding of C3b to biological targets of complement.?J Exp Med. 152:1102-14. Daha, MR, Stuffers-Heiman M, Kijlstra, A and Vans ES LA. (1979) Isolation and?characterization of the third component of rat complement. Immunology. 36:63-70 |
| 来源 | Normal mouse serum from healthy animals of mixed gender |
产品规格参数
| 物理外观 | Frozen liquid |
|---|---|
| 储存缓冲液 | 10 mM sodium phosphate, 145 mM NaCl, pH 7.2 |
| 储存温度 | 避免反复冻融,-80℃储存 |
| 运输条件 | 超低温冰袋运输 |
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