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C3b (Cynomolgus monkey)
规格或纯度:
1.0 mg/ml, 0.22 μm filtered
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基本描述
| 产品名称 | C3b (Cynomolgus monkey) |
|---|---|
| 规格或纯度 | 1.0 mg/ml, 0.22 μm filtered |
| 产品介绍 |
Protein Purity ≥85% by SDS PAGE Extinction Coeff A280 nm = 0.974 at 1.0 mg/ml for pure C3b Molecular Weight 176,000 Da (2 chains) General Description Cynomolgus monkey C3b (cyno C3b) is derived from native purified cynomolgus?monkey C3 (cyno C3, CompTech #CY113) upon cleavage and release of C3a with the?alternative pathway C3 convertase. C3b is central to the function of all three pathways of?complement (Law, S.K.A. and Reid, K.B.M. (1995)). Initiation of each pathway?generates proteolytic enzyme complexes (C3 convertases) which are bound to the target?surface. These enzymes cleave a peptide bond in C3 releasing the anaphylatoxin C3a and? activating C3b. For a brief time (~60 μs) this nascent C3b is capable of reacting with and?covalently coupling to hydroxyl groups on the target surface. Carbohydrates are the? favored target, but protein hydroxyls and amino groups also react. This process of?tagging the target surface with C3b is called opsonization. The reactive site in nascent?C3b is a thioester (Tack B.J., et al. (1980); Pangburn M.K. and MüllerEberhard H.J.?(1980)) and C3b is linked to the target through a covalent ester bond (an amide bond is?formed if C3b is attached to amino groups). Most of the C3 activated during complement?activation never attaches to the surface because its thioester reacts with water forming?fluid phase C3b which is rapidly inactivated by factors H and I forming iC3b. Surfacebound C3b is necessary in all three pathways for efficient activation of C5 and formation?of C5b-9 complexes that lyse the target cell membrane. Surface-bound C3b and its?breakdown products iC3b and C3d are recognized by numerous receptors on lymphoid?and phagocytic cells which use the C3b ligand to stimulate antigen presentation to cells?of the adaptive immune system. The end result is an expansion of target-specific B-cell?and T-cell populations. Physical Characteristics & Structure Cynomolgus monkey C3b is composed of two disulfide-linked chains. Analysis?of purified cyno C3b by SDS/polyacrylamide gel electrophoresis under non-reduced?conditions shows the mobility of cyno C3b to be similar to that of human C3b (176,000?daltons). Under reduced conditions, the migration of the alpha prime chain (α’-chain) of?cyno C3b is similar to that of human C3b alpha prime chain (101,000 daltons) while the?beta chain migrates slightly ahead of the human C3b beta chain (75,000 daltons).The concentration of purified cyno C3b is determined by using the calculated?extinction coefficient of cyno C3 based on its amino acid sequence using ProtParam and?assumes all pairs of Cys residues form cystines (i.e. a pair of cystine molecules are joined?by a disulfide bond). Function The biological functions of C3b are described under human C3b.? Assays Functional activity is determined by cleavage of cyno C3b by the human protease?factor I in the presence of the cofactor human factor H. This assay is very convenient as it? only requires factor H and I and SDS gels to analyze the cleavage of the alpha prime?chain of C3b in 67,000 and 43,000 Da fragments.? Precautions/Toxicity/Hazards This protein is purified from animal serum and therefore precautions appropriate?for handling any animal blood-derived product must be used. References Law, S.K.A. and Reid, K.B.M. (1995) Complement 2nd Edition (ISBN 0199633568)?Oxford University Press, Oxford. Tack BF, Harrison RA, Janatova J, Thomas ML, Prahl JW. (1980) Evidence for presence?of an internal thiolester bond in third component of human complement. Proc Natl Acad? Sci U S A. 77:5764-8. Pangburn M.K. and Müller-Eberhard H.J. (1980) Relation of putative thioester bond in? C3 to activation of the alternative pathway and the binding of C3b to biological targets of?complement. J Exp Med. 152:1102-14. |
| 来源 | Normal cynomolgus serum from healthy animals of mixed gender |
产品规格参数
| 物理外观 | Frozen liquid |
|---|---|
| 储存缓冲液 | 10 mM sodium phosphate, 145 mM NaCl, pH 7.2 |
| 储存温度 | 避免反复冻融,-80℃储存 |
| 运输条件 | 超低温冰袋运输 |
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