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限制性内切酶
规格或纯度:
医药级, ≥95%
有货
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| 规格或纯度 | 医药级, ≥95% | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 英文名称 | BsaI | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 单位定义 | 在 37℃、pH7.5 的条件下，1 小时内使 1μg 的 λDNA（HindIII 消化、无 Dcm 甲基化修饰）完全消化定义为 1 个活性单位。 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 储存温度 | -20°C储存 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 运输条件 | 超低温冰袋运输 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 产品介绍 |
?产品描述 限制性内切酶简称限制酶，是一类可识别特定的脱氧核苷酸序列，并对每条链中特定部位的两个脱氧核糖核苷酸之间的磷酸二酯键进行切割的核酸内切酶。限制酶是“限制-修饰系统”的重要组成部分，其生物学作用主要是保护宿主免受外来 DNA的感染，现被广泛应用于基因定位与克隆、基因结构研究、DNA 序列分析与测定、基因合成等各个领域。BsaI 来源于嗜热脂肪芽孢杆菌，是一种常用的限制性核酸内切酶。? 本产品是利用重组蛋白质生产技术获得限制酶 BsaI，采用药用规格原辅料生产，并严格控制宿主蛋白质残留、核酸残留等，符合 GMP 规范的产品生产与质量管理规程，保障生产过程及所有原辅料可追溯。? 产品用途 分子克隆；基因分型；Southern 杂交；限制性片段长度多态性(RFLP)。? 产品特点 1. 本产品具有强特异性，可针对其识别的 DNA 序列进行特异性切割，识别序列如下： ? ? 5,...GGTCTC(N)1↓...3, ? ? 3,...CCAGAG(N)5↑...5,? 2. 甲基化修饰影响： 受 CpG 甲基化影响，序列完全重叠，剪切阻断； 受 Dcm 甲基化影响，序列完全重叠，剪切阻断；? 受 EcoBI 甲基化影响，序列完全重叠，剪切可能受阻；? 不受 Dam 甲基化影响，序列无重叠，剪切不会受阻。? 保存体系 10 mM Tris-HCl; 300 mM NaCl; 1 mM DTT; 0.1 mM EDTA; 0.5mg/ml HSA; 50% Glycerol; pH 7.4 at 25℃。? 注意事项 BsaI 对某些甲基化修饰敏感，使用时注意酶切底物无 BsaI 敏感的甲基化修饰。 质量要求
遵循以下规范生产 1.ISO 9001:2015, certified facility。 2.《GMP 附录-细胞治疗产品》国家药品监督管理局。 3.《人用基因治疗总论-中国药典 2020》国家药典委。 4.USP Chapter <1043>, Ancillary Materials for Cell, Gene, and Tissue-Engineered Products 用于细胞治疗，基因治疗和组织工程产品中的辅料。 5.USP Chapter <92>, Growth Factors and Cytokines Used in Cell Therapy Manufacturing 细胞治疗产品生产过程中细胞因子和生长因子。 6. Ph. Eur. General Chapter 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products 用于生产细胞或基因治疗药物的生物来源原料。 Product Description Restriction endonucleases are abbreviated as restriction enzymes, which are a type of nucleic acid that can recognize a specific deoxynucleotide sequence and cleave the phosphodiester bond between two deoxyribonucleotides in a specific part of each chain. Dicer. Restriction enzymes are an important part of the "restriction-modification system". Its biological function is mainly to protect the host from foreign DNA infection. It is now widely used in gene location and cloning, gene structure research, DNA sequence analysis and determination, and gene Synthesis and other fields. BsaI is derived from Bacillus stearothermophilus and is a commonly used restriction endonuclease. This product uses recombinant protein production technology to obtain restricted enzyme BsaI. It is produced with medicinal raw materials and excipients, and strictly controls host protein residues, nucleic acid residues, etc., conforms to GMP standard product production and quality management procedures, and guarantees the production process and all raw materials Traceable. Product Usage Molecular cloning; Genotyping; Southern hybridization; Restriction fragment length polymorphism (RFLP). Features 1. This product has strong specificity and can specifically cut the DNA sequence it recognizes. The recognition sequence is as follows: ? ? 5,...GGTCTC(N)1↓...3, ? ? 3,...CCAGAG(N)5↑...5, 2. Influence of methylation modification: ? ? Affected by CpG methylation, the sequence is completely overlapped and cut off; ? ? Affected by Dcm methylation, the sequence is completely overlapped, and shearing is blocked; ? ? Affected by EcoBI methylation, the sequence completely overlaps, cutting may be blocked; ? ? It is not affected by Dam methylation, the sequence has no overlap, and the cutting will not be hindered. Preservation system 10 mM Tris-HCl; 300 mM NaCl; 1 mM DTT; 0.1 mM EDTA; 0.5mg/ml HSA; 50% Glycerol; pH 7.4 at 25°C. Precautions BsaI is sensitive to some methylation modifications. When using it, make sure that the enzyme digestion substrate does not have BsaI-sensitive methylation modifications. Quality requirements
Follow the following specifications for production 1. ISO 9001:2015, certified facility. 2. "GMP Appendix-Cell Therapy Products" State Drug Administration. 3. "General Introduction to Human Gene Therapy-Chinese Pharmacopoeia 2020" National Pharmacopoeia Commission. 4. USP Chapter <1043>, Ancillary Materials for Cell, Gene, and Tissue-Engineered Products are used as excipients in cell therapy, gene therapy and tissue engineering products. 5. USP Chapter <92>, Growth Factors and Cytokines Used in Cell Therapy Manufacturing Cytokines and growth factors used in the production of cell therapy products. 6. Ph. Eur. General Chapter 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products. |
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