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生物素
Goat Anti-Rabbit IgG H&L (Biotin)

Goat Anti-Rabbit IgG H&L (Biotin)

Secondary antibody, Goat Anti-Rabbit IgG H&L（Biotin）

规格或纯度: Secondary Antibody; Goat Anti-Rabbit IgG H&L (Biotin); WB, ELISA, IF

应用: WB,ELISA
亚型: Goat IgG

有货

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
Ab176442-100μg	100μg	期货
Ab176442-1mg	1mg	期货

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基本信息

产品名称	Goat Anti-Rabbit IgG H&L (Biotin)
规格或纯度	Secondary Antibody; Goat Anti-Rabbit IgG H&L (Biotin); WB, ELISA, IF
特异性	Rabbit IgG
交叉反应	No cross reactivity with mouse IgG, rabbit IgG, and human IgG
免疫原	Rabbit IgG

产品属性

克隆	Polyclonal
Format	Whole IgG
亚型	Goat IgG
SDS-PAGE	150kDa
纯化方法	Antigen affinity purified
物理外观	Liquid
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	1.0 mg/mL
储存温度	2-8°C储存,避光,避免反复冻融
运输条件	冰袋运输
稳定性与储存	Store at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

应用

Application	Notes
WB	1/10000-1/200000
ELISA	1:10000-1:200000

图片

Western Blot: Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442)

Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) at 1/40000 dilution
SA-HRP:1:20000

Predicted band size: 29 kDa
Observed band size: 31 kDa
Exposure time: 7.0 s

ELISA: Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442)

Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) - ELISA
Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) was tested by direct ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.