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Goat Anti-Human IgG (TRITC)

Secondary antibody, Goat Anti-Human IgG (TRITC)
规格或纯度: Secondary Antibody; Goat Anti-Human IgG (TRITC); IF, Flow
  • 应用: IF
有货

库存信息

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库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
Ab175836-100μg 100μg 现货 Stock Image
Ab175836-1mg 1mg 现货 Stock Image

基本信息

产品名称 Goat Anti-Human IgG (TRITC)
规格或纯度 Secondary Antibody; Goat Anti-Human IgG (TRITC); IF, Flow
特异性 Human IgG

产品属性

克隆 Polyclonal
Format Whole IgG
纯化方法 Antigen affinity purified
物理外观 Liquid
储存缓冲液 Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂 0.05% Proclin300
浓度 1.0 mg/mL
储存温度 避光,-20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

应用

Application Notes
IF

1:1000

图片

Goat Anti-Human IgG (TRITC) (Ab175836) - IF
IF analysis of EGFR (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Necitumumab (anti-EGFR) (Ab169264) at 1 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (TRITC) (Ab175836) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

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