| 产品名称 | Goat Anti-Human IgG (HRP) |
|---|---|
| 规格或纯度 | Secondary Antibody; Goat Anti-Human IgG (HRP); ELISA, WB, IHC |
| 特异性 | Human IgG |
| 克隆 | Polyclonal |
|---|---|
| Format | Whole IgG |
| 纯化方法 | Antigen affinity purified |
| 物理外观 | Liquid |
| 储存缓冲液 | Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300. |
| 防腐剂 | 0.05% Proclin300 |
| 浓度 | 1.0 mg/mL |
| 储存温度 | 避光,-20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Store in the dark. |
| Application | Notes |
|---|---|
| ELISA | 1:10000-1:100000 |
| WB | 1:10000-1:100000 |
Goat Anti-Human IgG (HRP) (Ab175835)- ELISA
Goat Anti-Human IgG (HRP) (Ab175835) was tested by direct ELISA. The plate was coated with different amounts of Human IgG (Ab170213). A serial dilution of Goat Anti-Human IgG (HRP) (Ab175835) was used as the secondary antibody.
Goat Anti-Human IgG (HRP) (Ab175835)- ELISA
Cross-reactivity of Goat Anti-Human IgG (HRP) (Ab175835) was tested by direct ELISA . The wells were coated with the indicated IgG at 0.5 μg/mL (100 μL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. Goat Anti-Human IgG (HRP) (Ab175835) at 1/10000 dilution was used as the primary antibody.
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