| 产品名称 | Goat Anti-Human IgG (Biotin) |
|---|---|
| 规格或纯度 | Secondary Antibody; Goat Anti-Human IgG (Biotin); ELISA, WB, IF, Flow |
| 特异性 | Human IgG |
| 克隆 | Polyclonal |
|---|---|
| Format | Whole IgG |
| 纯化方法 | Antigen affinity purified |
| 物理外观 | Liquid |
| 储存缓冲液 | Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300. |
| 防腐剂 | 0.05% Proclin300 |
| 浓度 | 1.0 mg/mL |
| 储存温度 | 2-8°C储存,避光,避免反复冻融 |
| 运输条件 | 冰袋运输 |
| 稳定性与储存 | Store at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light. |
| Application | Notes |
|---|---|
| Flow |
1/200 - 1/1000
|
| WB | 1/10000-1/200000 |
| IF | 1:1000 |
| ELISA | 1:10000-1:200000 |
Goat Anti-Human IgG (Biotin) (Ab175832) - ELISA
Goat Anti-Human IgG (Biotin) (Ab175832) was tested by direct ELISA. The plate was coated with different amounts of Human IgG (Ab170213). A serial dilution of Goat Anti-Human IgG (Biotin) (Ab175832) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.
Goat Anti-Human IgG (Biotin) (Ab175832) - ELISA
Cross-reactivity of Goat Anti-Human IgG (Biotin) (Ab175832) was tested by direct ELISA. The wells were coated with the indicated IgG at 0.5 μg/mL (100 μL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. Goat Anti-Human IgG (Biotin) (Ab175832) at 1/10000 dilution was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.
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