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荧光素
Goat Anti-Mouse IgG H&L (FITC)

Goat Anti-Mouse IgG H&L (FITC)

Secondary antibody, Goat Anti-Mouse IgG H&L (FITC)

规格或纯度: Secondary Antibody; Goat Anti-Mouse IgG H&L (FITC); IF, Flow

应用: IF,Flow

有货

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
Ab156254-100μg	100μg	现货
Ab156254-1mg	1mg	现货

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基本信息

产品名称	Goat Anti-Mouse IgG H&L (FITC)
规格或纯度	Secondary Antibody; Goat Anti-Mouse IgG H&L (FITC); IF, Flow
特异性	Mouse IgG
交叉反应	No cross reactivity with rabbit IgG, and human
免疫原	Mouse IgG

产品属性

克隆	Polyclonal
Format	Whole IgG
纯化方法	Antigen affinity purified
物理外观	Liquid
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	1.0 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

应用

Application	Notes
IF	1:1000
Flow	1/200-1/2000

图片

IF: Goat Anti-Mouse IgG H&L (FITC) (Ab156254)

Goat Anti-Mouse IgG H&L (FITC) (Ab156254) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L (FITC) (Ab156254)

Goat Anti-Mouse IgG H&L (FITC) (Ab156254) - Flow Cytometry
Flow analysis of beta Actin (red line) in Jurkat cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/5000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.