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二抗
生物素
Goat Anti-Mouse IgG H&L (Biotin)

Goat Anti-Mouse IgG H&L (Biotin)

Secondary antibody, Goat Anti-Mouse IgG H&L（Biotin）

规格或纯度: Secondary Antibody; Goat Anti-Mouse IgG H&L (Biotin); WB, ELISA, IF, Flow

应用: WB,IF,ELISA

有货

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
Ab156253-100μg	100μg	现货
Ab156253-1mg	1mg	现货

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基本信息

产品名称	Goat Anti-Mouse IgG H&L (Biotin)
规格或纯度	Secondary Antibody; Goat Anti-Mouse IgG H&L (Biotin); WB, ELISA, IF, Flow
特异性	Mouse IgG
交叉反应	No cross reactivity with rabbit IgG, and human IgG.
免疫原	Mouse IgG
阳性对照	ELISA: Mouse IgG

产品属性

克隆	Polyclonal
Format	Whole IgG
纯化方法	Antigen affinity purified
物理外观	Liquid
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	1.0 mg/mL
储存温度	2-8°C储存,避光,避免反复冻融
运输条件	冰袋运输
稳定性与储存	Store at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Avoid repeated freeze/thaw cycles. Protect from light.

应用

Application	Notes
WB	1/10000-1/200000
IF	1:1000
ELISA	1/20000-1/400000

图片

IF: Goat Anti-Mouse IgG H&L (Biotin) (Ab156253)

Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at a dilution of 1/200 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

ELISA: Goat Anti-Mouse IgG H&L (Biotin) (Ab156253)

Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - ELISA
Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.