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SMAD9 Antibody

功能和特点

规格或纯度: pAb; Rabbit anti Human SMAD9 Antibody; WB, IHC, IF, ICC; Unconjugated
简短描述: Rabbit polyclonal to SMAD9
反应种属: Human

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货号 (SKU)	包装规格	是否现货
Ab128246-10μl (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请三次您选择的不同货号试用装产品，尽享探索与享受！	10μl	期货
Ab128246-20μl	20μl	现货
Ab128246-50μl	50μL	现货
Ab128246-100μl	100μL	现货
Ab128246-1ml	1ml	期货

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Accession#:O15198 Gene ID:4093 SMAD9

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基本信息

产品名称	SMAD9 Antibody
规格或纯度	pAb; Rabbit anti Human SMAD9 Antibody; WB, IHC, IF, ICC; Unconjugated
特异性	SMAD9
反应种属	Human
免疫原	Recombinant SMAD9 expressed in E.coli (AA 226~459)
阳性对照	WB: HeLa, Hep G2, HEK293 and A549 cell lysates. IHC: Human cerebrum, glioma, kidney, liver cancer, heart tissues. IF/ICC: Hep G2 cells.
偶联标记	Unconjugated

产品属性

克隆	Polyclonal
Format	Whole IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified + Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4.
防腐剂	0.02% Sodium azide
浓度	Lot by Lot
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

应用

Application	Notes
IHC	5-20 μg/mL
WB	0.01-2 μg/mL
IF/ICC	5-20μg/mL

图片

Western Blot: SMAD9 Antibody (Ab128246)

SMAD9 Antibody (Ab128246) - Western Blot
All lanes: SMAD9 Antibody (Ab128246) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 48, 63 kDa
Exposure time: 0.8 s

IF/ICC: SMAD9 Antibody (Ab128246)

SMAD9 Antibody (Ab128246) - IF/ICC
FITC staining on IF
Samples: Human Hep G2 Cells
Primary Ab: 20 μg/mL SMAD9 Antibody (Ab128246)

IHC: SMAD9 Antibody (Ab128246)

SMAD9 Antibody (Ab128246) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 10 μg/mL SMAD9 Antibody (Ab128246)
Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SMAD9 Antibody (Ab128246)

SMAD9 Antibody (Ab128246) - IHC
DAB staining on IHC
Samples: Human Glioma Tissue
Primary Ab: 10 μg/mL SMAD9 Antibody (Ab128246)
Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SMAD9 Antibody (Ab128246)

SMAD9 Antibody (Ab128246) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 μg/mL SMAD9 Antibody (Ab128246)
Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SMAD9 Antibody (Ab128246)

SMAD9 Antibody (Ab128246) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 μg/mL SMAD9 Antibody (Ab128246)
Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.