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ha tag
HA tag Mouse mAb

HA tag Mouse mAb

功能和特点

规格或纯度: mAb (4G3); Mouse anti HA tag Antibody; WB, IF, ICC; Unconjugated
简短描述: Mouse monoclonal (4G3) to HA tag
亚型: Mouse IgG1

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货号 (SKU)	包装规格	是否现货
Ab106776-10μl (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请三次您选择的不同货号试用装产品，尽享探索与享受！	10μl	期货
Ab106776-50μl	50μL	现货
Ab106776-100μl	100μL	现货
Ab106776-1ml	1ml	期货

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HA tag Tag

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基本信息

产品名称	HA tag Mouse mAb
规格或纯度	mAb (4G3); Mouse anti HA tag Antibody; WB, IF, ICC; Unconjugated
特异性	HA
免疫原	Synthetic peptide HA-tag.
阳性对照	WB: Over-expressed HA-tagged protein in HEK293T cell lysate. IF/ICC: HEK293T cells, transfected with pcDNA3.1-HA-Flag-p62 #PPL00549-2b.
偶联标记	Unconjugated

产品属性

克隆	Monoclonal
克隆号	4G3
Format	Whole IgG
亚型	Mouse IgG1
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH 7.2
防腐剂	0.02% Sodium azide
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

应用

Application	Notes
WB	1/1000 - 1/2000
IF/ICC	1/1000

图片

Western Blot: HA tag Mouse mAb (Ab106776)

HA tag Mouse mAb (Ab106776) - Western Blot
All lanes: HA tag Mouse mAb (Ab106776) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 75 kDa
Observed band size: 77 kDa
Exposure time: 30.0 s

Western Blot: HA tag Mouse mAb (Ab106776)

HA tag Mouse mAb (Ab106776) - Western Blot
All lanes: HA tag Mouse mAb (Ab106776) at 1/1000 dilution
Samples: Recombinant Human HA-Ubiquitin Protein (rp170435) at 20 ng per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 11 kDa
Observed band size: 10 kDa
Exposure time: 30.0 s

IF/ICC: HA tag Mouse mAb (Ab106776)

HA tag Mouse mAb (Ab106776) - IF/ICC
IF image of HA tag Mouse mAb (Ab106776) stained HEK293T cells, transfected with pcDNA3.1-HA-Flag-p62. The cells were 4% para-formaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1 h to permeabilise the cells and block non-specific pro-tein-protein interactions. The cells were then incubated with the HA tag Mouse mAb (Ab106776) (1/200) and the antibody Flag-tag pAb(1/200) at 5 μg/mL overnight at 4°C. The secondary anti-body (Red) was Goat anti-Mouse IgG (H+L)-TRITC and the secondary antibody (Green) was Goat anti-Rabbit IgG (H+L)-FITC used at 1/1000 dilution for 1 h. Hoechst33342 was used to stain the cell nuclei (blue).