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aladdin?594 Click-iT EdU 通用款细胞增殖检测试剂盒(红色荧光)

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货号 (SKU) 包装规格 是否现货 价格 数量
A598375-2T 2-20T 期货 Stock Image
A598375-10T 10-100T 期货 Stock Image
A598375-50T 50-500T 期货 Stock Image
A598375-100T 100-1000T 期货 Stock Image

基本描述

英文名称 Aladdin ? 594 click it edu universal cell proliferation detection kit (red fluorescence)
储存温度 避光,-20°C储存
运输条件 超低温冰袋运输
产品介绍

产品介绍:

细胞增殖检测是评估细胞健康程度、遗传毒性及抗肿瘤药物效果的基础实验手段。检测细胞增殖最精确的方法是 BrdU法。EdU法检测试剂盒是BrdU法的革命性突破。EdU(5-乙炔基-2’-脱氧尿苷)是一种嘧啶类似物,在DNA合成期整合入DNA双链。EdU法检测基于“点击”反应,一种由铜催化的叠氮化合物和炔烃作用发生共价反应,形成共价键。 本试剂盒中,EdU含有炔烃,aladdin? 488/555/594/647A Azide染料含有叠氮化合物。点击法的EdU标记增殖快速有效,易于使用。BrdU方法需要DNA变性(如酸变性、热变性或者用DNase消化)暴露出BrdU,从而方便BrdU抗体结合;而EdU法只需多聚甲醛固定和Triton X-100促渗就可以使检测试剂进入细胞,只需少量的叠氮化染料即可非常有效地标记出整合的EdU。 本试剂盒包含EdU法检测所需要的所有组分,可以用于体外培养细胞的增殖检测。


产品参数:

YF? 488 Azide:495/519 nm;YF? 555 Azide:555/565 nm;YF?594 Azide:590/617 nm YF? 647A Azide:650/670 nm;Hoechst 33342:350/461 nm(bound to DNA)


注意事项:

1. 使用前请将产品瞬时离心至管底,再进行后续实验。 2. 荧光染料均存在淬灭问题,实验操作时请尽量注意避光,以减缓荧光淬灭。 3. Click-iT EdU缓冲液添加物溶液最好现配现用,以保证最佳结果。 4. 为了您的安全和健康,请穿实验服并戴一次性手套操作。


应用范围:

细胞增殖检测(细胞成像流式通用款)

Product introduction:

Cell proliferation detection is a basic experimental method to evaluate the health of cells, genotoxicity and the effect of antitumor drugs. The most accurate method to detect cell proliferation is the BrdU method. Edu detection kit is a revolutionary breakthrough of BrdU method. Edu (5-ethynyl-2 '- deoxyuridine) is a pyrimidine analog that integrates into the DNA duplex during DNA synthesis. Edu detection is based on the "click" reaction. A copper catalyzed azide reacts covalently with alkynes to form covalent bonds. In this kit, edu contains alkynes, Aladdin ? 488 / 555/594/647a azide dyes contain azide compounds. The edu labeling proliferation of click method is rapid and effective, and easy to use. BrdU method requires DNA denaturation (such as acid denaturation, thermal denaturation or digestion with DNase) to expose BrdU, so as to facilitate BrdU antibody binding; The edu method only needs paraformaldehyde fixation and Triton X-100 penetration to make the detection reagent enter the cells, and only a small amount of azide dye is needed to label the integrated edu very effectively. This kit contains all components required for edu method detection, and can be used for proliferation detection of cultured cells in vitro.


Product parameters:

YF ? 488 azide:495 / 519 nm; YF ? 555 azide:555 / 565 nm; YF ? 594 azide:590/617 nm YF ? 647a azide:650 / 670 nm; Hoechst 33342:350/461 nm (bound to DNA)


Matters needing attention:

1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. fluorescent dyes have quenching problems. Please try to avoid light during experimental operation to slow down fluorescence quenching. 3. click it edu buffer additive solution should be prepared and used immediately to ensure the best results. 4. for your safety and health, please wear experimental clothes and disposable gloves.


Scope of application:

Cell proliferation detection (cell imaging flow universal)

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