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化学和生化试剂
生化试剂
生物缓冲液
10-乙酰基-3,7-二羟基吩噁嗪（Ampliflu Red）

10-乙酰基-3,7-二羟基吩噁嗪（Ampliflu Red）

高灵敏度，稳定的过氧化物酶荧光底物。过氧化氢探针。

规格或纯度: ≥98.0% (HPLC),用于荧光分析

CAS编号: 119171-73-2
分子式: C₁₄H₁₁NO₄
分子量: 257.241
PubChem编号: 167453

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货号 (SKU)	包装规格	是否现货	价格	数量
A131064-5mg	5mg	现货
A131064-25mg	25mg	现货
A131064-100mg	100mg	现货

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基本描述

规格或纯度	≥98.0% (HPLC),用于荧光分析
英文名称	Ampliflu Red
别名	10-乙酰基-3,7-二羟基吩噁嗪\|ADHP\|荧光红染料
英文别名	10-Acetyl-3,7-dihydroxyphenoxazine\|119171-73-2\|1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone\|ADHP\|Amplex red reagent\|1-(3,7-dihydroxyphenoxazin-10-yl)ethanone\|Amplex red\|MFCD00467861\|Ethanone, 1-(3,7-dihydroxy-10H-phenoxazin-10-yl)-\|n-acetyl-3,7-dihydrox
生化机理	高度敏感，稳定的过氧化物酶荧光底物，用作过氧化氢探针。
储存温度	2-8°C储存,充氩
运输条件	冰袋运输
备注	如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。
产品介绍	Fluorogenic substrate for peroxidase;Ampliflu Red is used for the following applications: Quantification of inflammatory mediators;Determination of free and esterified cholesterol contents; Evaluation of compounds as substrates for GABA-AT compounds. We are pleased to introduce our serieal Amplite? fluorogenic substrates to address this unmet need. Our Amplite? ADHP is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2?detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or?related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol,?L-glutamate, amino acids, etc. Although ADHP exhibits very high sensitivity for detecting peroxidate activity either in a fluorimetric or colorimetric mode, it requires higher pH to reach its maximum fluorescence. We are pleased to introduce our Amplite? ADAP that has better water solubility and stronger fluorescence than ADHP. Addtionally, our Amplite???is the only fluorogenic peroxidase substrate that generates fluorescent precipitates upon the peroxidase-catalyzed oxidation. Fluorogenic substrate for peroxidase;Ampliflu Red is used for the following applications: Quantification of inflammatory mediators;Determination of free and esterified cholesterol contents; Evaluation of compounds as substrates for GABA-AT compounds. We are pleased to introduce our serieal Amplite? fluorogenic substrates to address this unmet need. Our Amplite? ADHP is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2?detection is at least one order of magnitude more sensitive than the commonly used scopoletin?assay for H2O2. Because H2O2is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or?related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol,?L-glutamate, amino acids, etc. Although ADHP exhibits very high sensitivity for detecting peroxidate activity either in a fluorimetric or colorimetric mode, it requires higher pH to reach its maximum fluorescence. We are pleased to introduce our Amplite? ADAP that has better water solubility and stronger fluorescence than ADHP. Addtionally, our Amplite???is the only fluorogenic peroxidase substrate that generates fluorescent precipitates upon the peroxidase-catalyzed oxidation.

名称和标识符

IUPAC Name	1-(3,7-dihydroxyphenoxazin-10-yl)ethanone
INCHI	InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
InChi Key	PKYCWFICOKSIHZ-UHFFFAOYSA-N
Canonical SMILES	CC(=O)N1C2=C(C=C(C=C2)O)OC3=C1C=CC(=C3)O
PubChem CID	167453
分子量	257.241

化学和物理性质

敏感性	对光线和空气敏感
熔点	> 250 °C

安全和危险性（GHS）

象形图	Harmful
信号词	Warning
危险声明	H302: Harmful if swallowed
预防措施声明	P501,P264,P270,P330,P301+P317

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