91高清自产国产拍,无码免费A级毛片大全,日本 欧美 国产中文字幕,99re热视频这里只有精品视频首页

Buffer Reference Center

Buffer Reference Center

  • pH Ranges of Selected Biological Buffers Chart (25 °C, 0.1 M)

  • Tris or Trizma??Buffer Preparation – pH vs. Temperature

  • Phosphate Buffer Preparation – 0.2 M solution

  • Citric Acid – Na2HPO4?Buffer Preparation, pH 2.6-7.6

  • Citric Acid – Sodium Citrate Buffer Preparation, pH 3.0-6.2

  • Sodium Acetate – Acetic Acid Buffer Preparation, pH 3.7-5.6

  • Na2HPO4?– NaH22PO4?Buffer Preparation, pH 5.8-8.0 at 25 °C

  • Imidazole (glyoxaline) – HCl Buffer Preparation, pH 6.2-7.8 at 25 °C

  • Sodium Carbonate – Sodium Bicarbonate Buffer Preparation, pH 9.2-10.8

  • Buffer Preparation Formulas and Equations

CHOOSING THE RIGHT BIOLOGICAL BUFFER

Choose a buffer based on your pH requirements as well as the pKa, a measure of acid strength that accounts for pH, concentration, and temperature. Regulatory or purity needs for your exact application should also be considered. The following tables can help you navigate preparation of many common buffer solutions by pH and pKa.


Buffer Level

Buffer grade indicates the quality and impurity levels appropriate for different uses. We provide six grades of buffers indicated for general lab use, final pharmaceutical formulation and manufacturing, and applications in between that may need trace metal testing or materials of a specified purity.


USEFUL PH RANGES OF SELECTED BIOLOGICAL BUFFERS CHART (25 °C, 0.1 M)


Buffers

Useful pH

Range
pKa(20℃)pKa(25℃)pKa(37℃)Reagent Grade
Higher Grade
MES5.5-6.76.166.15.97M105074M274288
Bis-Tris5.8-7.2n/a6.56.36B105639B113072
ADA6.0-7.26.656.596.46A108964A108965
ACES6.1-7.56.886.786.54A100327A100328
PIPES6.1-7.56.86.766.66P105090P105092
P105093P105091
MOPSO6.2-7.6n/a6.96.75M113008n/a
Bis-Tris-Propane6.3-9.5n/a6.8,9.0n/aB299299n/a
B105640
BES6.4-7.87.177.096.9B105116B105117
B105115
MOPS6.5-7.97.287.27.02M105135M274252
M105133
TES6.8-9.27.57.47.16T105113T105114
HEPES6.8-8.27.557.487.31H109407H109408
H109406
DIPSO7.0-8.2n/a7.67.35B113022n/a
MOBS6.9-8.3n/a7.6n/aM120630n/a
TAPSO7.0-8.2n/a7.67.39T113029n/a
Tris7.0-9.08.28.067.72T110600T110602
T110599
T110598
T274227
T274250
T110601
HEPPSO7.1-8.5n/a7.86.66H113003n/a
POPSO7.2-8.5n/a7.87.63P105272n/a
TEA7.3-8.3n/a7.8n/aT108151n/a
T108154
EPPS7.3-8.7n/a8n/aH110899H110901
H110900
Tricine7.4-8.88.168.057.8T100351T118067
T100350
Gly-gGly7.5-8.9n/a8.2n/aG105429G119491
G119492
G119491
Bicine7.6-9.08.358.268.04B110905B110906
HEPBS7.6-9.0n/a8.3n/aH113002n/a
TAPS7.7-9.18.498.48.18T108961T108963
T108962
AMPD7.8-9.7n/a8.8n/aA106026n/a
TABS8.2-9.6n/a8.9n/aT329718n/a
AMPSO8.3-9.7n/a99.1A120640n/a
CHES8.6-10.09.559.499.36C110897n/a
C110898
CAPSO8.9-10.3n/a9.69.43C105213n/a
AMP9.0-10.5n/a9.7n/aA140833n/a
A100592
CAPS9.7-11.110.5610.410.02C105209n/a
C105210
CABS10.0-11.4n/a10.7n/aC120628n/a


TRIS BUFFER PREPARATION TABLE – PH VS. TEMPERATURE


pH at Temperature     g/L for 0.05M Solution
5℃ 25℃ 37℃ Tris-HCl Tris base
7.76 7.2 6.91 7.02 0.67
7.89 7.3 7.02 6.85 0.8
7.97 7.4 7.12 6.61 0.97
8.07 7.5 7.22 6.35 1.18
8.18 7.6 7.3 6.06 1.39
8.26 7.7 7.4 5.72 1.66
8.37 7.8 7.52 5.32 1.97
8.48 7.9 7.62 4.88 2.3
8.58 8 7.71 4.44 2.65
8.68 8.1 7.8 4.02 2.97
8.78 8.2 7.91 3.54 3.34
8.88 8.3 8.01 3.07 3.7
8.98 8.4 8.1 2.64 4.03
9.09 8.5 8.22 2.21 4.36
9.18 8.6 8.31 1.83 4.65
9.28 8.7 8.42 1.5 4.9
9.36 8.8 8.51 1.23 5.13
9.47 8.9 8.62 0.96 5.32
9.56 9 8.7 0.76 5.47

?

PHOSPHATE BUFFER PREPARATION TABLE – 0.2 M SOLUTION


Potassium Sodium Phosphate Dibasic Heptahydrate 23℃ pH Potassium Phosphate Monobasic Sodium Phosphate 23℃ pH
Phosphate Monobasic Anhydrous g/L     Anhydrous g/L Dibasic Heptahydrate
22.4 3.49 5.7 10.8 29.51 6.9
22.08 4.29 5.8 9.36 32.73 7
21.6 5.37 5.9 7.92 35.95 7.1
21.05 6.6 6 6.72 38.63 7.2
20.4 8.05 6.1 5.52 41.31 7.3
19.56 9.93 6.2 4.56 43.46 7.4
18.6 12.07 6.3 3.84 45.07 7.5
17.64 14.22 6.4 3.12 46.68 7.6
16.44 16.9 6.5 2.52 48.55 7.7
15 20.12 6.6 2.04 49.09 7.8
13.56 23.34 6.7 1.68 49.89 7.9
12.24 26.29 6.8 1.27 50.81 8

?

CITRIC ACID – NA2HPO4 BUFFER PREPARATION, PH 2.6–7.61?

Citric acid monohydrate, C6H8O7 ? H2O, MW 210.14; 0.1 M contains 21.01 g/L. Na2HPO4, MW 141.98; 0.2 M contains 28.40 g/L, or Na2HPO4 ? 2H2O, MW 178.05; 0.2 M contains 35.61 g/L.


x mL 0.1 M-citric acid and y mL 0.2 M-Na2HPO4?mixed


pH X mL 0.1M-citric acid Y mL 0.2M-Na2HPO4
2.6 89.1 10.9
2.8 84.15 15.85
3 79.45 20.55
3.2 75.3 24.7
3.4 71.5 28.5
3.6 67.8 32.2
3.8 64.5 35.5
4 61.45 38.55
4.2 58.6 41.4
4.4 55.9 44.1
4.6 53.25 46.75
4.8 50.7 49.3
5 48.5 51.5
5.2 46.4 53.6
5.4 44.25 55.75
5.6 42 58
5.8 39.55 60.45
6 36.85 63.15
6.2 33.9 66.1
6.4 30.75 69.25
6.6 27.25 72.75
6.8 22.75 77.25
7 17.65 82.35
7.2 13.05 86.95
7.4 9.15 90.85
7.6 6.35 93.65

?

CITRIC ACID – SODIUM CITRATE BUFFER PREPARATION, PH 3.0-6.21

Citric acid monohydrate, C6H8O7?? H2O, MW 210.14; 0.1 M contains 21.01 g/L. Trisodium citrate dihydrate, C6H5O7Na3??2H2O, MW 294.12; 0.1 M contains 29.41 g/L.


x mL 0.1 M-citric acid and y mL 0.1 M-trisodium citrate mixed


pH X mL 0.1M-citric acid Y mL 0.1M-trisodium citrate
3 82 18
3.2 77.5 22.5
3.4 73 27
3.6 68.5 31.5
3.8 63.5 36.5
4 59 41
4.2 54 46
4.4 49.5 50.5
4.6 44.5 55.5
4.8 40 60
5 35 65
5.2 30.5 69.5
5.4 25.5 74.5
5.6 21 79
5.8 16 84
6 11.5 88.5
6.2 8 92


SODIUM ACETATE – ACETIC ACID BUFFER PREPARATION, PH 3.7–5.61?

Sodium acetate trihydrate, CH3COONa ? 3H2O, MW 136.09; 0.2 M contains 27.22 g/L.


x mL 0.2 M-NaOAc and y mL 0.2 M-HOAc mixed


pH,18℃ X mL 0.2M-NaOAc Y mL 0.2M-HOAc
3.7 10 90
3.8 12 88
4 18 82
4.2 26.5 73.5
4.4 37 63
4.6 49 51
4.8 59 41
5 70 30
5.2 79 21
5.4 86 14
5.6 91 9

?

NA2HPO4?– NAH2PO4?BUFFER PREPARATION, PH 5.8-8.0 AT 25 °C1?

Na2HPO4? 2H2O, MW 178.05; 0.2 M contains 35.61 g/L. Na2HPO4 ? 12H2O, MW 358.22; 0.2 M contains 71.64 g/L. NaH2PO4 ? H2O, MW 138.01; 0.2 M contains 27.6 g/L. NaH2PO4 ? 2H2O, MW 156.03; 0.2 M contains 31.21 g/L.


x mL 0.2 M-Na2HPO4, y mL 0.2 M-NaH2PO4; diluted to 100 mL with H2O


pH,25℃ X mL 0.2M-Na2HPO4 Y mL 0.2M-NaH2PO4
5.8 4 46
6 6.15 43.85
6.2 9.25 40.75
6.4 13.25 36.75
6.6 18.75 31.25
6.8 24.5 25.5
7 30.5 19.5
7.2 36 14
7.4 40.5 9.5
7.6 43.5 6.5
7.8 45.75 4.25
8 47.35 2.65

?



IMIDAZOLE (GLYOXALINE) – HCL BUFFER PREPARATION, PH 6.2–7.8 AT 25 °C1

Imidazole,C3H4N2,, MW 68.08


25 mL 0.2 M-imidazole (13.62 g/L), x mL 0.2 M-HCl, diluted to 100 mL with H2O


pH, 25℃ X mL 0.2M-HCl
6.2 21.45
6.4 19.9
6.6 17.75
6.8 15.2
7 12.15
7.2 9.3
7.4 6.8
7.6 4.65
7.8 3


SODIUM CARBONATE – SODIUM BICARBONATE BUFFER PREPARATION, PH 9.2-10.81

Na2CO3 ? 10H2O, MW 286.2; 0.1 M contains 28.62 g/L. NaHCO3, MW 84.0; 0.1 M contains 8.40 g/L.


x mL 0.1 M-Na2CO3and y mL 0.1 M-Na2HCO3mixed


20℃ 37℃
X mL 0.1M-Na2CO3? Y mL 0.1M-NaHCO3,
9.2 8.8
9.4 9.1
9.5 9.4
9.8 9.5
9.9 9.7
10.1 9.9
10.3 10.1
10.5 10.3
10.8 10.6


BUFFER PREPARATION FORMULAS AND EQUATIONS

Percentage by weight (w/v)

(% buffer desired / 100) × final buffer volume (mL) = g of starting material needed.


Molar Solutions

desired molarity × formula weight × solution final volume (L) = grams needed


Henderson-Hasselbach Equation



项目号产品名称规格CAS编号包装
M274288

MES Free Acid Monohydrate

Ultra Pure Grade
145224-94-8100g/250g/500g/1kg/12x100g/12x250g/12x500g
M105074

MES monohydrate

≥99% (T)145224-94-825g/100g/500g/2.5kg
B105639

BIS-TRIS

98%6976-37-025g/100g/500g
B113072

BIS-TRIS

用于细胞和昆虫细胞培养, ≥98% (HPLC)6976-37-025g/100g/500g
A108965

N-(2-Acetamido)iminodiacetic acid

Ultra Pure, ≥99.0% (T)26239-55-425g/100g
A108964

N-(2-Acetamido)iminodiacetic acid

98%26239-55-425g/100g/500g
A100327 N-(2-Acetamido)iminodiacetic acid
99%7365-82-425g/100g/500g
A100328 N-(2-Acetamido)iminodiacetic acid
超纯级,≥99.5% (T)7365-82-425g/100g
P105090

1,4-Piperazinediethanesulfonic acid(PIPES)

99%5625-37-625g/100g/500g
P105093 1,4-Piperazinediethanesulfonic acid(PIPES)
99.5%5625-37-625g/100g/500g
P105092 1,4-Piperazinediethanesulfonic acid(PIPES)
for cell culture,≥99%5625-37-6
25g/100g/500g/1kg/2.5kg
M113008

3-(N-Morpholino)-2-hydroxy-1-propanesulfonic acid

99%68399-77-925g/100g/500g/1kg
B299299

BIS-TRIS propane

98%64431-96-55g/25g/100g/500g
B105640

BIS-TRIS propane

99%64431-96-5
5g/25g/100g/500g
B105115

BES

≥99%(T) 10191-18-1
25g/100g/500g/1kg/2.5kg
B105116 BES
≥99.5%(T)10191-18-1
25g/100g/250g
B105117 BES
用于分子生物学,≥99.5%(T)10191-18-1
25g/100g/500g
T105114

TES

超纯级,≥ 99.5%(T)7365-44-825g/100g/500g
T105113 TES
99%7365-44-8
5g/25g/100g/500g
H109406

HEPES

99%7365-45-925g/100g/500g/1kg/2.5kg
H109408 HEPES
用于分子生物学,≥99.5%(T)7365-45-9
5g/25g/100g/500g
H109407 HEPES
≥99.5%(T)7365-45-9
25g/100g/500g/1kg/5kg
B113022

3-[Bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid

98%68399-80-425g/100g/500g
M120630

4-(N-Morpholino)butanesulfonic Acid

99%115724-21-525g/100g
T113029

N-[Tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropanesulfonic acid

99%68399-81-525g/100g/500g
T274250

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)

超纯级77-86-1500g/1kg/5kg/10kg/12x500g
T110600 Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
ACS, ≥99.8%77-86-1
25g/100g/500g
T110601 Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
分子生物学级,≥99.9%(T)77-86-1
250g/1kg
T110599 Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
标准缓冲物质, ≥99.9% (titration)77-86-1
100g/500g/1kg/5kg/10kg
T110602 Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
细胞培养级,≥99.9%(T)77-86-1
100g/500g/1kg/5kg
T274227 Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
蛋白质组级77-86-1
500g/1kg/12x500g/4x1kg
T110598 Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
EP, USP, 已细胞培养测试, ≥99.9% (T)77-86-1
100g/500g/2.5kg
H113003

4-(2-Hydroxyethyl)piperazine-1-(2-hydroxypropanesulfonic acid) monohydrate

99%68399-78-05g/25g/100g
P105272

Piperazine-1,4-bis(2-hydroxypropanesulfonic Acid) Dihydrate

99%68189-43-525g/100g/500g/2.5kg
T108151

Triethanolamine

AR102-71-6100ml/500ml/2.5L
T108150 Triethanolamine
Standard for GC, ≥99.5% (GC)102-71-6
5ml
T108153 Triethanolamine
色谱级,>99.0% (GC)102-71-6
100ml/500ml
T108154 Triethanolamine
≥99.0% (GC)102-71-6
100ml/500ml/2.5L
H110899

HEPPS

≥99.0%(T)16052-06-55g/25g/100g/500g
H110901 HEPPS
用于细胞培养,≥99.5%(T)16052-06-5
25g/100g/500g
T100351

Tricine

99.5%5704-04-125g/100g/500g
T118067 Tricine
for cell culture,≥99.5%(T)5704-04-1
25g/100g/500g/1kg
T100350 Tricine
99%5704-04-1
25g/100g/500g
G105429

Gly-Gly

99%556-50-35g/25g/100g/500g
G119491 Gly-Gly
超纯级, ≥99.5% (NT)556-50-3
10g/50g
G119492 Gly-Gly
细胞培养级, ≥99% (titration)556-50-3
25g/100g/500g
B110906

N,N-Bis(2-hydroxyethyl)glycine

超纯级,≥99.5% (T) 150-25-4
25g/100g/500g
B110905 N,N-Bis(2-hydroxyethyl)glycine
99%150-25-425g/100g/500g
T108962TAPS≥99.5%(T)29915-38-625g/100g/500g/5kg
T108963 TAPS
用于细胞培养级,≥99.5%(T)29915-38-6
25g/100g/500g
T108961 TAPS
99%29915-38-6
5g/25g/100g/500g
A106026

2-Amino-2-methyl-1,3-propanediol

98%115-69-525g/100g
A120640AMPSO99%68399-79-15g/25g/100g
C110898CHES≥99.0%(T)103-47-95g/25g/100g/500g
C110897CHES≥99.5%(T)103-47-925g/100g
C105213CAPSO99%73463-39-525g/100g/500g/2.5kg
A100592

2-Amino-2-methyl-1-propanol

90%124-68-525ml/100ml/500ml/2.5L
A140833 2-Amino-2-methyl-1-propanol
≥95%124-68-5
25ml/100ml/500ml/2.5L
C105209CAPS≥98.0%1135-40-625g/100g/500g/1kg/5kg
C105210CAPS超纯级, ≥99.0% (HPLC)1135-40-6
25g/100g/500g

References

1. Dawson R, Elliot D, Elliot W, Jones KM. 1986. Data for Biochemical Research. 3rd ed.. Oxford Science Publ..


Aladdin:https://www.aladdinsci.com

标签:

91高清自产国产拍,无码免费A级毛片大全,日本 欧美 国产中文字幕,99re热视频这里只有精品视频首页

品牌简介

{转码主词}